CRISPR/Cas9 genome editing is widely used due to its simplicity and versatility, and CRISPR technology has been adapted to many applications other than genome editing. As a leader in gene synthesis and genome editing, Microbiosci provides proven CRISPR products, services and resources to help utilize the power of
CRISPR genome editing for research.
CRISPR stands for clustered regularly spaced short palindrome repeats. CRISPR sequences were originally identified in the genome of E. coli and have been found to be part of an RNA-based adaptive immune system to target and destroy genetic parasites at the DNA level. CRISPR-related protein (Cas) is an endonuclease that cleaves foreign DNA and integrates it into the host genome. Only when there is a pro-spacer adjacent motif (PAM) around the target sequence of the invading DNA, the cleavage will occur, thus ensuring highly accurate targeting. Researchers studying CRISPR modified it to be used as a tool for genetic modification of the target host genome. CRISPR/Cas9 has recently become a popular genome editing tool due to its simplicity and versatility. Researchers studying CRISPR modified it to be used as a tool for genetic modification of the target host genome.
Genome editing technology can be operated at the genome level, where DNA is replaced, deleted, or inserted into the organism. Classic genome editing methods depend on homology-directed repair and stem cell totipotency to facilitate the modification of individual genes. The 2007 Nobel Prize in Physiology or Medicine was awarded for the discovery of the principle of introducing specific genetic modifications in mice using embryonic stem cells. The traditional HDR method has the disadvantages of low efficiency, high technical requirements and high cost, which severely limit its application in large-scale genome manipulation research. In 2013, the discovery of type II CRISPR-Cas9 system promoted the development of precise genetic modification. This new RNA-mediated DNA editing method opens up new ways for the application of genome editing technology in animal model construction, treatment of genetic diseases and agricultural breeding.
The CRISPR-Cas9 system precisely targets and edits specific target DNA sequences through a programmable mechanism, and provides a general method for establishing transgenic animal models. Although mouse models have been widely used,
CRISPR-Cas9 gene editing methods have been established in many other animal models. CRISPR-Cas9 can generate precise genome editing of a specific mouse genome by injecting Cas9 mRNA and directly introducing RNA (sgRNA) into the mouse embryo, with an efficiency of 100%, thereby generating a new mouse model. CRISPR-Cas9-mediated targeting and editing promote the generation of knock-in and knock-out mouse models, which greatly reduces time and resource consumption compared with traditional methods.
Although CRISPR-Cas9 has been widely used as a research tool, a particularly exciting future direction is the development of CRISPR-Cas9 as a therapeutic technology for the treatment of genetic diseases. In addition, CRISPR-Cas9 technology provides exciting possibilities for creating crop varieties with ideal traits without introducing foreign DNA.
CRISPR-Cas9 is an emerging technology that allows precise genome modification without introducing foreign genes. This transformative tool is expected to revolutionize biological research and expand our ability to correct the genetic causes of many diseases.
Microbiosci has mastered the science and technology of CRISPR gene editing. We obtained highly certified cell lines from our collection and used single-guide RNA (sgRNA) designed to guide Cas9 to the target area to introduce disease-related mutations. The parental cell line was co-transfected with sgRNA and Cas9. Transfected cells are classified into single cells and expanded for testing. Then strictly screen the isogenic cell clones after gene editing. Microbiosci has established a CRISPR-Cas9 gene/genome editing platform that can provide a one-stop solution for the CRISPR-Cas9 project. The services we can provide include: CRISPR-Cas9 sgRNA design, CRISPR-Cas9 sgRNA library design and genome editing.