Profacgen, with an organized team of scientists with extensive experience in the study of protein-protein interaction, recently updated its pull down assay service to help efficiently and specifically verify the predicted protein-protein interactions or identify novel interactions.
Protein-protein interactions (PPIs) are established when protein molecules physically contact each other through hydrogen or hydrophobic bonds, van der Waals forces, ionic forces, or even covalent bonds. This phenomenon underlies a wide range of fundamental cellular events, such as signal transduction, molecular transport and various metabolic pathways. PPIs also underlie many diseases. Therefore, PPIs have been extensively studied in the field of bioscience and medical research.
Protein pull-down assay is an in vitro affinity purification method that uses bait proteins to enrich proteins that interact with bait proteins. It can be used to confirm existing protein-protein interactions found by other techniques, or for preliminary screening to identify novel protein-protein interactions.
Now, pull down assay services at Profacgen mainly include:
Construction of recombinant expression plasmid
Bait protein expression and purification
Identification of protein-protein interaction by Western Blot or MS
Data retrieval and bioinformatics analysis
Results submission
Both bait protein and prey protein can be obtained from cell lysis, protein expression system or in vitro transcription and translation system through its carefully designed experimental scheme, according to the official speaker from Profacgen.
Featuring as: fully customized high quality service, stringent quality control and consistent results, short turn-around time, best price in the market, and detailed experimental reports, pull down assay services at Profacgen is sure to help with the detection of possible interacting proteins of certain target proteins at ease.
“Besides pull down assay, we have also established in our lab a series of assays to help with your research, including: Yeast Two-Hybrid Screening, Membrane-based Yeast Two-Hybrid Screening, Surface Plasmon Resonance (SPR), Co-Immunoprecipitation (Co-IP), Bio-layer Interferometry (BLI), Isothermal Titration Calorimetry, etc.” commented Crystal, the chief marketing staff at Profacgen.
More detailed information about the updated pull down assay at Profacgen can be viewed here: https://www.profacgen.com/pull-down-assay.htm.
About Profacgen
Profacgen provides process development and cGMP manufacturing services for biological active ingredients. We have ready access to state-of-the-art biopharmaceutical product development and manufacturing facilities and our staff scientists have demonstrable expertise in all aspects of biopharmaceutical manufacturing, from facility design and operation to the ability to provide best advice relating to product development.