RNA Solutions is a platform that offers services for the revolutionizing study of RNA, targeting both coding and non-coding RNAs of different physiological functions. Scientists from CD Genomics are ready to help detect various types of RNA via meticulous and integrative approaches. CD Genomics is proud to launch the Digital RNA Sequencing technology, a next-generation sequencing-based method that eliminates sequence-dependent PCR bias by barcoding RNA molecules prior to amplification.
After the inception of next-generation DNA sequencing, RNA sequencing (RNA-Seq) was developed, which provided a great tool for transcriptome profiling. However, conventional RNA-Seq still has room for improvement. For example, conventional RNA-Seq is hampered by inaccuracy from exponential PCR amplification and sequence-dependent bias during sample preparation and sequencing.
“CD Genomics has developed a simple strategy for mitigating these complications, allowing truly digital RNA-Seq. Following reverse transcription, a large set of barcode sequences are added in excess, and nearly every cDNA molecule is uniquely labeled by random attachment of barcode sequences. After PCR, we applied deep sequencing to read barcodes and cDNA sequences. Rather than counting the number of reads, RNA abundance is measured based on the number of unique barcode sequences observed for a given cDNA sequence,” said the Chief Scientist of CD Genomics.
“We optimized the barcodes to be unambiguously identifiable, even in the presence of multiple sequencing errors. This method allows counting with single-copy resolution despite sequence-dependent bias and PCR-amplification noise and is analogous to digital PCR but amendable to quantifying a whole transcriptome. This technique is very useful for single-cell analysis which requires high accuracy and sensitivity,” she added.
Learn about the key features of digital RNA sequencing technology offered by CD Genomics:
- Low starting material: 100 ng of starting materials achieve the equivalent sequencing result of 1 µg, which is more suitable for rare and precious samples
- UMI technology: Adding UMI to each cDNA fragment can remove PCR amplification bias, which truly reflect the abundance of transcript expression and achieve accurate and unbiased quantification
- Improve transcriptome sequencing quality: Accurately analyze expression levels and screen differential expression, and accurately lock RNA editing, alternative splicing, and SNP sites
- Suitable for multiple RNA-Seq
CD Genomics provides a full digital RNA sequencing service package including sample quality control, library construction, deep sequencing, raw data quality control, DEG analysis, and customized bioinformatics analysis. CD Genomics will continue to develop innovative methods with enhanced sensitivity to detect more complex and low-abundance transcripts while reducing the cost per study and making it more accessible to all laboratories.
About CD Genomics
CD Genomics enjoys a high reputation for sequencing, microarray analysis, library construction and genotyping, providing reliable services to pharmaceutical and biotechnology companies as well as academia and government agencies.
Contact
Address: Shirley, NY 11967, USA
Email: contact@cd-genomics.com