Creative Bioarray, one of the leading biotechnology companies focuses on developing high-quality products and services in order to accelerate research in the field of biopharma, drug development and life science industry. Recently, Creative Bioarray announced the release of its cell immortalization services and kits that can be applied to extend the replicative capacity of target cells and further support potential drug development.
Based on an experienced team of scientists and a well-designed technology platform, Creative Bioarray provides cell immortalization services and has been able to successfully immortalize cells from any species and any tissue with functions desired by customers. As a leading manufacturer of primary cells, stem cells and immune cells, Creative Bioarray can provide starting material from any species and any tissue. In addition, the professional team is capable to establish an isolation protocol and then conducting custom isolation for the clients.
Currently, Creative Bioarray has developed several HT®
Cell Immortalization Kits for immortalizing mammalian cells in culture:
Viral genes, such as EBV, HPV-16 E6/7 genes, and SV40 T antigen, are used to induce immortalization. In most cases, viral genes are immortalized by inactivating tumor suppressor genes (p53, Rb, etc.) that can induce a replicative senescence state in cells.
SV40 T antigen has been shown to be the simplest and most reliable reagent for transforming many different cell types in culture, and the mechanism of SV40 T antigen in cell immortalization has been intensively studied. Recent studies have also shown that the SV40 T antigen can induce telomerase activity in infected cells.
A recently discovered method of cell immortalization is through the expression of TERT, especially for cells that are most affected by telomere length. This protein is inactive in most somatic cells, but when hTERT is exogenously expressed, cells are able to maintain sufficient telomere length to avoid replicative senescence. Analysis of several telomerase-immortalized cell lines has confirmed that cells maintain a stable genotype and retain key phenotypic markers.
Co-expression of the hTERT catalytic subunit with p53 or RB siRNA immortalizes primary human ovarian epithelial cells, providing a more realistic normal cell model with well-defined genetic background.
“Creative Bioarray can provide you with an integrated platform of immortalized human cell lines to meet the needs of any related research project.” said Hannah Cole, the marketing director of Creative Bioarray, she also added, “Our service team can work with you to develop and execute flexible custom-designed experiments to meet your unique needs and goals.”
About Creative BioarrayCreative Bioarray is dedicated to offering customers innovative biotechnology products and services for research use to greatly enhance and drive innovation and standards in science. As a well-recognized industry leader with more than 10 years of experience and in-house expert support, Creative Bioarray has already countenanced research all around the world.