Pronalyse is a subdivision of Creative Proteomics, an integrated CRO company with rich experience in providing drug development services. Based on years of experience supporting protein characterization research, Creative Proteomics Pronalyse introduces hydrogen-deuterium exchange mass spectrometry to help scientists accelerate the study of advanced protein structures.
HDX-MS (hydrogen-deuterium exchange mass spectrometry) technology is a mass spectrometry technique for studying the spatial conformation of proteins. The principle of this technique is to immerse protein molecules in a deuterium-containing aqueous solution and exchange amide hydrogens of the protein molecule with deuterium atoms from the solution at different rates. This process depends on the protein configuration to a certain extent, and the hydrogen-deuterium exchange rate of protein fragments is determined by mass spectrometry so as to obtain information regarding the spatial structure of the protein as well as interpret the degree of activity of different sites.
Hydrogen-deuterium exchange mass spectrometry can quickly elucidate the amino acid sequence of the protein surface in a natural solution, so it has a wide range of applications in the study of protein epitopes, also known as antibody-binding sites. Since the hydrogen-deuterium exchange mass spectrometry relies on the principle of hydrogen-deuterium atom exchange, the backcrossing of hydrogen-deuterium atoms will greatly impact the accuracy of experimental results. At present, the backcrossing of hydrogen and deuterium atoms is mainly controlled by shortening the liquid mass analytical period and controlling the temperature and pH to achieve the lowest backcrossing reaction coefficient.
For advanced protein structure studies, hydrogen-deuterium exchange mass spectrometry (HDX-MS) has become an indispensable technology for biotechnology companies. Technological innovations in HDX-MS-related LC-MS, automation, and informatics have enabled it to evolve from scientific research to a robust industrial tool for protein drug discovery.
Creative Proteomics’ HDX-MS technology is suitable for various applications, including the study of protein-drug binding, protein-protein interactions, epitope mapping, protein conformation and dynamics, aggregation due to dosage forms, and changes in configuration positioning caused by stability, etc.
“The HDX technology enabled mass spectrometry to open a new window for the study of biomolecular dynamics by providing the relative deuterium content of different configurations or positions in proteins. Recent continuous development by combining HDX with mass spectrometry has revealed advanced capability and made it an important tool for studying the higher-order structure (HOS) dynamics of biotherapeutics. Not to mention the increasingly favored industry and regulatory trends in elucidating the configuration and relationships between biomolecules, making HDX a key tool of choice in the field of biotherapeutic analysis,” stated Pronalyse’s project manager.
About Creative Proteomics
Creative Proteomics is an innovative contract research organization that focuses on using advanced equipment platforms and optimized detection technology to help researchers around the world reduce time and costs. The company has a proprietary laboratory information management system to strengthen laboratory quality monitoring and management, ensuring the efficiency and security of customer data.
Contact
Address: Shirley, NY 11967, USA
Email: contact@creative-proteomics.com
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